metI [2017-07-14 10:03:07]

O-succinylhomoserine lyase (L-cysteine, H2S, methanethiol, elimination)

locus: BSU11870

pI: 5.02

mw: 41.55 kDa

protein length: 373 aa

gene length: 1119 bp

function: biosynthesis of methionine

product: O-succinylhomoserine lyase (L-cysteine, H2S, methanethiol, elimination)

essential: no

synonyms: yjcI

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.10|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine]

Gene

Coordinates: 1,258,492 → 1,259,613

Expression and Regulation

Operons

• genes: [gene|04DC792180FDC5E7916F2DBB2EC2C34369202FE0|metI]-[gene|1C0D38F23D3A2CAA1EB057A92D6F571D0D2AA724|metC]
  description: [Pubmed|11832514]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|11832514], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [regulon|S-box|S-box]: transcription termination/ antitermination, the [SW|S-box] [SW|riboswitch] binds S-adenosylmethionine resulting in termination, in [regulon|S-box|S-box]

  regulation

  • induced by methionine starvation ([SW|S-box]) [Pubmed|10094622]
  • the [SW|S-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

Biological materials

Mutant

• MGNA-B298 (yjcI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1297 NBRP B. subtilis, Japan]
• 1A941 ( ''metI''::''spec''), [Pubmed|11832514], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A941&Search=1A941 BGSC]

References

• 19258532,11832514,10094622,11948165,12107147,18039762